Dear Team,
I notice we have a Half-life Data information in Tractability session. I am wondering where we can find more information about “Half-life Data”. Are we able to get “Half-life Data” for all the human targets?
Thanks.
Shicheng
Hi Shicheng,
The half-life assessment is derived from the PROTACtable Genome workflow, which gets the half-life assessments from Mathieson et al.. Quoting from the PROTACtable genome paper:
The half-life data bucket (7) indicates the presence of experimental data on protein half-life. Knowledge of the rate at which a protein is naturally turned over is relevant to the use of a PROTAC designed to artificially accelerate this process, as a very short protein half-life may limit the effective utility of a PROTAC compound. A recent study from Mathieson et al. (see Supplementary Data 2 in their paper) describes the use of SILAC-based proteomics to determine protein half-lives ranging from 10 to >1,000 h in a variety of cell types (B cells, natural killer cells, hepatocytes, monocytes and mouse embryonic neurons). In our PROTAC workflow, we capture both minimal and maximal half-lives across the different cell types studied.